what is the minimum concentration of nicotine that can by detected in urine by the assay?

A spectrometric assay is designed to work with final concentrations of 0.005 to 0.020 mg/L of nicotine. If a 500 ml sample of urine is used and all of the nicotine present is concentrated into a final solution volume of 10.00 mL, what is the minimum concentration of nicotine that can by detected in…